Invitrogen electroporator ii manual

For technical support, email tech_support@www.doorway.ru For country-specific contact information, visit www.doorway.ru Page 2 GenePulser® II electroporator, we recommend using the following electroporator conditions: kV, Ω, 25 µF (see Note 2). 6. To the cells in the cuvette, add 1 ml of S.O.C. medium and transfer the. Instruction Manual: 1. Neon ® Kits The Neon ® Kits are used with the Neon ® Transfection Systems for efficient transfection of mammalian cells and areavailable separately from (page 40). The kits are available in two formats for electroporation of 10 µL and µL samples. BioRad GenePulserfi II electroporator, we recommend using the following electroporator conditions: kV, Ω, 25 µF (see Note 2). 7. To the cells in the cuvette, add 1 ml of S.O.C. medium and transfer the solution to a 15 ml snap-cap tube (e.g. FalconŽ tube). 8. Shake at rpm (37°C) for 1 hour.

User Manual EasySelect ™ Pichia Expression Kit For Expression of Recombinant Proteins Using pPICZ and pPICZα in Pichia pastoris Cat. no. K Rev. Date 18 June Manual part no. MAN Invitrogen Electroporator II Manual (www.doorway.ru). Briefly, a www.doorway.ru culturewas grown to an OD of –, then cells were harvested by centrifugation and washed twice in ice-cold water and once in ice-cold 10% glycerol by repeated centrifugation and suspension. At the final step the. Electroporation-competent cells were prepared as described in the Invitrogen Electroporator II Manual (www.doorway.ru). Briefly, a ml www.doorway.ru culture was grown to an OD of , then cells were harvested by centrifugation and washed twice in ice-cold water and once in ice-cold 10% glycerol by repeated centrifugation and suspension.

11 កក្កដា kits are available in two formats for electroporation of 10 µL Follow the instructions below to unpack the Neon® Transfection System. All usage must comply with product instructions. Includes: Neon electroporation device, pipette, pipette station, USB thumbdrive, manual and power cord. Here we report a protocol to regenerate these expensive tips as well as some other NeonTM kit accessories, thereby reducing the cost of electroporation at least.